Ecosystems In Fractured Aquifer Groundwater Can Treat Contamination

Groundwater is not often regarded as ecosystems and especially fractured aquifer systems are seen as organism free. Conventional tests show very little to no presence of micro-organisms in groundwater. However, these micro-organisms are ubiquitous and can be detected by using sophisticated methods. In this specific case study where petroleum hydrocarbon  contamination exists in a fractured rock aquifer, the presence of micro-organisms has been for years inferred by means of monitoring for secondary lines of evidence that prove attenuation of the contaminants, not only by means of dilution, adsorption or diffusion into the matrix, but through metabolism. The sampling evidence is clear that the preferential sequence of metabolism is taking place whereby electron acceptors are reduced as predicted for such biodegradation. Specifically sulphate is consumed and mostly manganese is reduced, with some iron reduction also being observed. Monitoring has shown that  groundwater recharge bringing in  new  nutrients effected increased biodegradation. In order to definitively identify the contribution made by micro-organisms, DNA testing was performed. The results support the secondary lines of evidence. Outside of the contaminated zone very low population numbers of organisms were detected in the groundwater. Inside the contaminated zone elevated population numbers were observed indicating that active biodegradation is taking place. Furthermore, the edges of the plume, where contaminant levels are mostly below detection, contained a more diverse population of micro-organisms than the central area. Conditions on the edge of the plume probably represent an ideal nutrient environment for the organisms as opposed to the high concentration source, which might be toxic to some organisms. Better understanding of the bio-dynamics of this fractured aquifer presents a unique opportunity to better manage and enhance the remediation of the contaminants. Possible strategies include the addition of nutrients when necessary and the cultivation of the naturally occurring organisms to augment the population. The data shows that aquifers are ecosystems even in fractured environments.