Multiple-tool approach of combining microbial markers with artificial and natural tracers to specify the origin of faecal contamination on karst groundwater catchment scale

Diverse tools exist to study the transfer of contamination from its source to groundwater and related springs. A backward approach, i.e. sampling spring water to determine the origin of contamination, is more complex and requires multiple information. Microbial source tracking (MST) using host-specific markers is one of the tools, which, however, has shown to be insufficient as a stand-alone method, particularly in karst groundwater catchments. A karst spring in the Swiss Jura Mountains was studied concerning the occurrence and correlation of a set of faecal indicators, including classical parameters and bacteroidal markers. Sporadic monitoring proved the impact on spring water quality, mainly during high water stages. Additional event-focused sampling evidenced a more detailed and divergent pattern of individual indicators. A multiple-tool approach, complementing faecal indicator monitoring with artificial tracer experiments and measuring natural tracers, could specify the origin of ruminant and human faecal contaminations. Natural tracers allowed for distinguishing between water components from the saturated zone, the soil/epikarst storage, or freshly infiltrated rainwater. Additionally, the breakthrough of injected dye tracers and their remobilization during subsequent recharge events were correlated with the occurrence of faecal markers. The findings hypothesize that human faecal contamination is related to septic tanks overflowing at moderate rainfall intensities. Linkage with vulnerability assessment and land-use information can finally better locate the potential point sources. Such a toolbox provides useful basics for groundwater protection and catchment management and insight into general processes governing the fate and transport of faecal contaminants in karst environments.

Presenter Name
M
Presenter Surname
Sinreich
Area
Switzerland
Conference year
2023