Determining The Mass Balance Of Synthetic Dna Tracer In Headwater Streams

The pollution of water resources has become a growing concern worldwide. Industrial, agricultural and domestic activities play a pivotal role in water resources pollution. The challenge faced by pollution   monitoring   networks   is   to   understand   the   spatial   and   temporal   distribution   of contaminants. In hydrology, tracers have become a critical research tool to investigate surface water and groundwater transport dynamics. Synthetic DNA (deoxyribonucleic acid) tracers are being used in hydrological research to determine source areas, where uniquely labelled DNA from each source area  is  identified.  The main  objective  of the  study  was to  determine  the mass  balance of  the synthetic DNA tracer in surface water streams. Furthermore, to gain knowledge on DNA adsorption and decay and determine whether DNA behaves as conservative tracer in the surface water streams. Understanding the adsorption and decay characteristics of synthetic DNA tracers may promote its robustness in hydrological research. In this study, field injection experiments using synthetic DNA were  carried  out,  the  DNA  tracer  was  injected  together  with  sodium  chloride  (salt)  and deuterium as conservative reference tracers. The purpose was to compute DNA mass balance calculations with reference to the two conservative tracers. In this study two different DNA markers were used, namely T22 and T23. Additionally, with each injection experiment a field batch experiment was carried out to determine DNA loss characteristics on the field. From our study, the DNA loss between the injection point and the first measurement was greater than 90%. Therefore, it was important to conduct additional laboratory batch experiments to explain DNA loss characteristics. However, the issue of the initial DNA loss remained unresolved. Laboratory batch experiments results allow us to conclude the following: the type of material used, filtering, ion concentration and water composition reduced DNA concentration. Moreover, initial DNA losses occurred and not DNA decay. From our experiments we concluded that DNA can be used for long-term tracer experiments, subsequently, limiting synthetic DNA mass balance determination of synthetic DNA as it is a reactive. Overall, we can conclude that DNA does not behave as a conservative tracer.